Services

Glycomics

CE - monosaccharides Lectin affinity separation - proteins Lectin affinity separation - peptides Multi-Lectin affinity separation - proteins Multi-Lectin affinity separation - peptides MALDI-MS - polyusaccharides (shotgun glycomics) LC-MS/MS - glycosylated peptides (Q-TOF / MALDI-TOF/TOF) Contact Yehia Mechref (ymechref@indiana.edu) for glycomics services.

Bottom-up Proteomics

LC-MS/MS - peptides (LCQ / XCT) - up to 240 minutes of RP gradient LC-IMS/MS/MS - peptides - up to 240 minutes of RP gradient

Other Proteomics Services

2-DE - proteins In-gel digestion - peptide mass fingerprinting In-gel digestion - LC-MS/MS Peptide mass fingerprinting (already digested) - MALDI-TOFMS MALDI tandem MS - TOF/TOF (per spectrum) In-solution trypsin digestion Database search - peptide mass fingerprinting Database search - MS/MS Contact Randy Arnold (rarnold@indiana.edu) for proteomics services.

Glycomics/Glycoproteomics/Proteomics Service request:

Researcher Name:
Email:
Phone:
Research Group:
	(Billing will be sent directly to research advisor.)
Account #:
Building / Room:
Street Address:
# Samples:
Analysis requested:
Type of sample:
	(Intact protein / trypsin digest / solution / lyophilized / etc.)
Sample volume:
Approximate concentration (mg/mL) or amount if solid:
Buffers in sample (w/ concentration):
Detergents in sample (w/ concentration):
Peptide/Protein identification requested:
Organism / source:
Other useful information (optional):
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THIS IS ONLY A REQUEST FOR INFORMATION; not a complete order form or a guarantee of labtime reservation. You will be contacted via email within 3 days of submission.

 

 

Manuscripts acknowledging NCGG Services Core

In Print

• J. Burnham, L. Gong and R. W. Hardy "Heterogeneous nuclear ribonuclear protein K interacts with Sindbis virus nonstructural proteins and viral subgenomic mRNA" Virology 2007, 367, 212-221.

Submitted / In Preparation

• M. Lawler, R. Arnold, and Y. V. Brun. "Identification of the processing site for the Caulobacter crescentus polar localization factor PodJ" - In preparation for Molecular Microbiology
• He, X. and C. Fuqua. Analysis of the quorum sensing regulon of Rhizobium sp. strain NGR234 reveals a novel mechanism for growth rate control. - Submitted to Journal of Bacteriology.
• Lee, T. Arnold, R and Feig, AL. "Protein Content of Hfq RNP Particles Varies as a Function of the ncRNA Incorporated Within it." - in preparation
• Li, Y, Weitzel, CS, Arnold, RJ, Oakley, MG: Identification of Interacting Regions within the Coiled Coil of the Eschericia Coli Structural Maintenance of Chromosomes Protein MukB. In preparation for J. Mol. Biol.